Protein Cybernetics develop applications and programs for investigating molecular events involved in protein functions. Our principle activities are aimed at the identification of proteins interacting in human cells during transitions between quiescence and growth, the entry into DNA replication, cell division and the arrest of proliferative growth and reprogramming into differentiation.
We have developed methods for identifying sub-femtomolar levels of unknown proteins. In addition we are capable of identifying protein phosphorylation events in proteins from 50-100 human cells.
Over the next few weeks we will bring these protocols onto this page concomitant with the release and deployment of our software programs to facilitate these challenges.
We hope you find them useful.
At present, our web site is still under construction. We are currently bringing three new approaches to protein phosphorylation identification to the web. Available as licensed, but free, versions will be compatible with Unix/Motif/X and OSX. Progams will also be available through the our web_based interface.
In the meantime you can contact us at our e-mail address: resolve@protein-cybernetics.com.
If you are not familiar with our company products. Phospepsort2006 and phospepsort2007 are predictive web based tools for estimating peptide mobility on 2 dimensional thin layer chromatographs (2D-TLC). Particularly adapted for the analysis of phosphorylated peptides using standardized markers. We have since developed TLC-analyzer for identifying unknown peptides in known protein sequences based on TLC mobility in both electrophoretic and chromatographic dimensions.
As of 2008, we have begun developing and licensing stand alone programs for predicting peptide electrophoretic mobility (emob), chromatographic mobility (rfcalc), both together (resolve). In addition digester which performs the same functions on complex peptides and proteins after predicting peptide cleavage.
Finally, we have now an experimental version of HPLC-calc. This final prediction software package applies the same HiLo algorithm used in TLC prediction in the determination of peptide elution profiles from a C18 HPLC column. Elution profiles are calculated for any 2 pH values between 1.9 and 9 and allow the identification of peaks derivd from any known peptide sequence. At 2 fixed pH values, the same algorithm can be applied to unknown proteins of human origin on the basis the initial mass, pI, enzymatic treatment and elution times.
